Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain.

A mixture of ananain (EC 3.4.22.31) and comosain purified from crude pineapple stem extract was found to contain numerous closely related enzyme forms. Chromatographic separation of the major enzyme forms was achieved after treatment of the mixture with thiol-modifying reagents: reversible modificat...

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Main Authors: Napper, A D, Bennett, S P, Borowski, M, Holdridge, M B, Leonard, M J, Rogers, E E, Duan, Y, Laursen, R A, Reinhold, B, Shames, S L
Format: Artigo
Sprog:Inglês
Udgivet: 1994
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137048/
https://ncbi.nlm.nih.gov/pubmed/8053898
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