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Purification and properties of uroporphyrinogen III synthase (co-synthase) from an overproducing recombinant strain of Escherichia coli K-12.

The Escherichia coli hemD gene, encoding the enzyme uroporphyrinogen III synthase (co-synthase), was cloned into multi-copy plasmids in E. coli cells that were used to generate strains producing up to 1000 times the concentration of the synthase in the wild-type. The enzyme was purified to homogenei...

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Bibliographische Detailangaben
Hauptverfasser: Alwan, A F, Mgbeje, B I, Jordan, P M
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1989
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1133594/
https://ncbi.nlm.nih.gov/pubmed/2557837
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