Modification of catalytically important carboxy residues in endoglucanase D from Clostridium thermocellum.

Endoglucanase D (EC 3.2.1.4; EGD) from Clostridium thermocellum is rapidly (k = 216 M-1.min-1) and almost completely (greater than 95%) inactivated with Woodward's reagent K (N-ethyl-5-phenylisoxazolium-3'-sulphonate). Spectrophotometric analysis at 340 nm reveals that eight carboxy residu...

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Autors principals: Tomme, P, van Beeumen, J, Claeyssens, M
Format: Artigo
Idioma:Inglês
Publicat: 1992
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1132783/
https://ncbi.nlm.nih.gov/pubmed/1637316
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