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Modification of catalytically important carboxy residues in endoglucanase D from Clostridium thermocellum.
Endoglucanase D (EC 3.2.1.4; EGD) from Clostridium thermocellum is rapidly (k = 216 M-1.min-1) and almost completely (greater than 95%) inactivated with Woodward's reagent K (N-ethyl-5-phenylisoxazolium-3'-sulphonate). Spectrophotometric analysis at 340 nm reveals that eight carboxy residu...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1992
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1132783/ https://ncbi.nlm.nih.gov/pubmed/1637316 |
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