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Determination of the order of substrate addition to MspI DNA methyltransferase using a novel mechanism-based inhibitor.

The cloning and overexpression of the MspI DNA methyltransferase as a functional fusion with glutathione S-transferase is described. The fusion enzyme retains full biological activity and has been used to investigate the interaction of substrates and inhibitors with MspI DNA methyltransferase. The f...

詳細記述

保存先:
書誌詳細
主要な著者: Taylor, C, Ford, K, Connolly, B A, Hornby, D P
フォーマット: Artigo
言語:Inglês
出版事項: 1993
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1132552/
https://ncbi.nlm.nih.gov/pubmed/8484730
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