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The pKa of the catalytic histidine residue of chloramphenicol acetyltransferase.

A catalytically essential histidine residue (His-195) of chloramphenicol acetyltransferase (CAT) acts as a general base in catalysis, abstracting a proton from the primary hydroxy group of chloramphenicol. The pKa of His-195 has been determined from the pH-dependence of chemical modification. Both m...

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Autors principals: Lewendon, A, Shaw, W V
Format: Artigo
Idioma:Inglês
Publicat: 1993
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1132376/
https://ncbi.nlm.nih.gov/pubmed/8439283
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