Extracellular-matrix degradation at acid pH. Avian osteoclast acid collagenase isolation and characterization.

Osteoclasts degrade bone matrix, which is mainly type I collagen and hydroxyapatite, in an acidic extracellular compartment. Thus we reasoned that osteoclasts must produce an acid collagenase. We purified this enzyme, a 31 kDa protein, from avian osteoclast lysates (in 100 mM acetate/1 mM CHAPS/1 mM...

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Detalhes bibliográficos
Main Authors: Blair, H C, Teitelbaum, S L, Grosso, L E, Lacey, D L, Tan, H L, McCourt, D W, Jeffrey, J J
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1132362/
https://ncbi.nlm.nih.gov/pubmed/8457215
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