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Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox

Directed evolution techniques allow us to genuinely mimic molecular evolution in vitro. To enhance this imitation of natural evolutionary processes on a laboratory scale in even more detail, we developed an in vitro method for the generation of random deletions and repeats. The pairwise fusion of tw...

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Detalhes bibliográficos
Main Authors: Pikkemaat, Mariël G., Janssen, Dick B.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC113230/
https://ncbi.nlm.nih.gov/pubmed/11937643
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