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Secondary structure prediction and in vitro accessibility of mRNA as tools in the selection of target sites for ribozymes

We have investigated the relative merits of two commonly used methods for target site selection for ribozymes: secondary structure prediction (MFold program) and in vitro accessibility assays. A total of eight methylated ribozymes with DNA arms were synthesized and analyzed in a transient co-transfe...

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Autori principali: Amarzguioui, Mohammed, Brede, Gaute, Babaie, Eshrat, Grøtli, Morten, Sproat, Brian, Prydz, Hans
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2000
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC113158/
https://ncbi.nlm.nih.gov/pubmed/11058107
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