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Charge-to-Alanine Mutagenesis of the Adeno-Associated Virus Type 2 Rep78/68 Proteins Yields Temperature-Sensitive and Magnesium-Dependent Variants

The adeno-associated virus type 2 (AAV) replication (Rep) proteins Rep78 and 68 (Rep78/68) exhibit a number of biochemical activities required for AAV replication, including specific binding to a 22-bp region of the terminal repeat, site-specific endonuclease activity, and helicase activity. Individ...

תיאור מלא

שמור ב:
הוצא לאור ב:J Virol
Principais autores: Gavin, Denise K., Young, Samuel M., Xiao, Weidong, Temple, Brenda, Abernathy, Corinne R., Pereira, Daniel J., Muzyczka, Nicholas, Samulski, Richard Jude
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology (ASM) 1999
נושאים:
Replication
גישה מקוונת:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC112978/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10516052/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.73.11.9433-9445.1999
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