Construction and Transposon Mutagenesis in Escherichia coli of a Full-Length Infectious Clone of Pseudorabies Virus, an Alphaherpesvirus

A full-length clone of the 142-kb pseudorabies virus (PRV) genome was constructed as a stable F plasmid in Escherichia coli. The clone, pBecker1, was colinear with PRV-Becker genomic DNA, lacking detectable rearrangements, deletions, or inversions. The transfection of pBecker1 into susceptible eukar...

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Bibliographic Details
Main Authors: Smith, Gregory A., Enquist, Lynn W.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1999
Subjects:
Pathogenesis and Immunity
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC112720/
https://ncbi.nlm.nih.gov/pubmed/10400733
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