Construction and Transposon Mutagenesis in Escherichia coli of a Full-Length Infectious Clone of Pseudorabies Virus, an Alphaherpesvirus

A full-length clone of the 142-kb pseudorabies virus (PRV) genome was constructed as a stable F plasmid in Escherichia coli. The clone, pBecker1, was colinear with PRV-Becker genomic DNA, lacking detectable rearrangements, deletions, or inversions. The transfection of pBecker1 into susceptible eukar...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Smith, Gregory A., Enquist, Lynn W.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Pathogenesis and Immunity
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC112720/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10400733/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.73.8.6405-6414.1999
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