Fast Screening Procedures for Random Transposon Libraries of Cloned Herpesvirus Genomes: Mutational Analysis of Human Cytomegalovirus Envelope Glycoprotein Genes

We have cloned the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome (BAC) in Escherichia coli. Here, we have subjected the HCMV BAC to random transposon (Tn) mutagenesis using a Tn1721-derived insertion sequence and have provided the conditions for excision of the...

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Detaylı Bibliyografya
Asıl Yazarlar: Hobom, Urs, Brune, Wolfram, Messerle, Martin, Hahn, Gabriele, Koszinowski, Ulrich H.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology 2000
Konular:
Structure and Assembly
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC112300/
https://ncbi.nlm.nih.gov/pubmed/10933677
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