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Poliovirus Requires a Precise 5′ End for Efficient Positive-Strand RNA Synthesis

Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5′ end, which are deleted during replication to generate authentic viral genomes. We removed those 5′-end extra nucleotides utilizing...

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Detalhes bibliográficos
Main Authors: Herold, Jens, Andino, Raul
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC112146/
https://ncbi.nlm.nih.gov/pubmed/10864650
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