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Plasmid pGS5 from the Hyperthermophilic Archaeon Archaeoglobus profundus Is Negatively Supercoiled

We present evidence that, in contrast to plasmids from other hyperthermophilic archaea, which are in the relaxed to positively supercoiled state, plasmid pGS5 (2.8 kb) from Archaeoglobus profundus is negatively supercoiled. This might be due to the presence of a gyrase introducing negative supercoil...

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Detalhes bibliográficos
Main Authors: López-García, Purificación, Forterre, Patrick, van der Oost, John, Erauso, Gaël
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC111383/
https://ncbi.nlm.nih.gov/pubmed/10940047
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