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Amplification of IgG VH and VL (Fab) from single human plasma cells and B cells

Amplification of human immunoglobulin has many potential applications such as analysis of clonality, isolation of immunogenic antigens and antigen-specific immunotherapy. Here we describe a method for amplification of human immunoglobulin heavy and light chains from single B lymphocytes or plasma ce...

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Bibliographic Details
Main Authors: Coronella, J. A., Telleman, P., Truong, T. D., Ylera, F., Junghans, R. P.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2000
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC110801/
https://ncbi.nlm.nih.gov/pubmed/11024187
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