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DNA binding properties in vivo and target recognition domain sequence alignment analyses of wild-type and mutant RsrI [N6-adenine] DNA methyltransferases

A genetic selection method, the P22 challenge-phage assay, was used to characterize DNA binding in vivo by the prokaryotic β class [N6-adenine] DNA methyltransferase M·RsrI. M·RsrI mutants with altered binding affinities in vivo were isolated. Unlike the wild-type enzyme, a catalytically compromised...

詳細記述

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書誌詳細
主要な著者: Szegedi, Sandra S., Gumport, Richard I.
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 2000
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC110778/
https://ncbi.nlm.nih.gov/pubmed/11024177
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