DNA binding properties in vivo and target recognition domain sequence alignment analyses of wild-type and mutant RsrI [N6-adenine] DNA methyltransferases

A genetic selection method, the P22 challenge-phage assay, was used to characterize DNA binding in vivo by the prokaryotic β class [N6-adenine] DNA methyltransferase M·RsrI. M·RsrI mutants with altered binding affinities in vivo were isolated. Unlike the wild-type enzyme, a catalytically compromised...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Szegedi, Sandra S., Gumport, Richard I.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2000
Schlagworte:	Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110778/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11024177/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.20.3972
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