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Psoralen photo-crosslinked mRNA–puromycin conjugates: a novel template for the rapid and facile preparation of mRNA–protein fusions

We describe the synthesis of a novel type of mRNA template and its use in the preparation of mRNA–protein fusions. A light-induced psoralen crosslinking reaction was used to attach a puromycin-containing oligonucleotide to the 3′-end of an mRNA template. The photo-crosslinked template was found to u...

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Sonraí Bibleagrafaíochta
Main Authors: Kurz, Markus, Gu, Ke, Lohse, Peter A.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Oxford University Press 2000
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC110755/
https://ncbi.nlm.nih.gov/pubmed/10982894
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