Psoralen photo-crosslinked mRNA–puromycin conjugates: a novel template for the rapid and facile preparation of mRNA–protein fusions
We describe the synthesis of a novel type of mRNA template and its use in the preparation of mRNA–protein fusions. A light-induced psoralen crosslinking reaction was used to attach a puromycin-containing oligonucleotide to the 3′-end of an mRNA template. The photo-crosslinked template was found to u...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2000
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110755/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10982894/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.18.e83 |
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