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Analysis of Gene Targeting and Intrachromosomal Homologous Recombination Stimulated by Genomic Double-Strand Breaks in Mouse Embryonic Stem Cells

To investigate the effects of in vivo genomic DNA double-strand breaks on the efficiency and mechanisms of gene targeting in mouse embryonic stem cells, we have used a series of insertion and replacement vectors carrying two, one, or no genomic sites for the rare-cutting endonuclease I-SceI. These v...

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Publicat a:Mol Cell Biol
Autors principals: Donoho, Greg, Jasin, Maria, Berg, Paul
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis 1998
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC108991/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9632791/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.18.7.4070
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