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Efficiency of Particle-Bombardment-Mediated Transformation Is Influenced by Cell Cycle Stage in Synchronized Cultured Cells of Tobacco
Plasmid DNA pB1221 harboring β-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G(2) phases gave 4 to 6 times higher transformation efficiency tha...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1991
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1081205/ https://ncbi.nlm.nih.gov/pubmed/16668589 |
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