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Methanococcus jannaschii Flap Endonuclease: Expression, Purification, and Substrate Requirements

The flap endonuclease (FEN) of the hyperthermophilic archaeon Methanococcus jannaschii was expressed in Escherichia coli and purified to homogeneity. FEN retained activity after preincubation at 95°C for 15 min. A pseudo-Y-shaped substrate was formed by hybridization of two partially complementary o...

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Bibliografiske detaljer
Main Authors: Rao, H. G. V., Rosenfeld, Amy, Wetmur, James G.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 1998
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC107589/
https://ncbi.nlm.nih.gov/pubmed/9765572
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