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Solubilization of Plant Membrane Proteins for Analysis by Two-Dimensional Gel Electrophoresis
A plasma membrane-enriched fraction prepared from barley roots was analyzed by two-dimensional gel electrophoresis. Four methods of sample solubilization were assessed on silver stained gels. When membranes were solubilized with 2% sodium dodecyl sulfate followed by addition of Nonidet P-40, gels ha...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1986
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1075430/ https://ncbi.nlm.nih.gov/pubmed/16664906 |
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