Mutational Analysis of the Transcriptional Regulator GcvA: Amino Acids Important for Activation, Repression, and DNA Binding
The GcvA protein is required for both glycine-mediated activation and purine-mediated repression of the gcvTHP operon. Random and site-directed PCR mutagenesis was used to create nucleotide changes in gcvA to identify residues of the protein involved in activation, repression, and DNA binding. Singl...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1998
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107511/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9733689/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.18.4865-4871.1998 |
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