Mutational Analysis of the Transcriptional Regulator GcvA: Amino Acids Important for Activation, Repression, and DNA Binding

The GcvA protein is required for both glycine-mediated activation and purine-mediated repression of the gcvTHP operon. Random and site-directed PCR mutagenesis was used to create nucleotide changes in gcvA to identify residues of the protein involved in activation, repression, and DNA binding. Singl...

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Bibliografische Detailangaben

Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Jourdan, Alissa D., Stauffer, George V.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1998
Schlagworte:	Genetics and Molecular Biology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107511/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9733689/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.18.4865-4871.1998
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