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Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

The success of long polynucleotide de novo synthesis is largely dependent on the quality and purity of the oligonucleotides used. Generally, the primary product of any synthesis reaction is directly cloned, and clones with correct products have to be identified. In this study, a novel strategy has b...

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書目詳細資料
Main Authors: Fuhrmann, Markus, Oertel, Wolfgang, Berthold, Peter, Hegemann, Peter
格式: Artigo
語言:Inglês
出版: Oxford University Press 2005
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1072809/
https://ncbi.nlm.nih.gov/pubmed/15800209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni058
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