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Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

The success of long polynucleotide de novo synthesis is largely dependent on the quality and purity of the oligonucleotides used. Generally, the primary product of any synthesis reaction is directly cloned, and clones with correct products have to be identified. In this study, a novel strategy has b...

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Detalhes bibliográficos
Main Authors: Fuhrmann, Markus, Oertel, Wolfgang, Berthold, Peter, Hegemann, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1072809/
https://ncbi.nlm.nih.gov/pubmed/15800209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni058
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