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Cloning and Physical Mapping of the EcoRI Fragments of the Giant Linear Plasmid SCP1
A cosmid library was constructed for the 350-kb giant linear plasmid SCP1 and aligned on a successive linear map. Only a 0.8-kb gap has remained uncloned in the terminal inverted repeats close to both ends. Partial digestion of the aligned cosmids with EcoRI and hybridization with the flanking fragm...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology
1998
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC107240/ https://ncbi.nlm.nih.gov/pubmed/9573173 |
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