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Cloning and Physical Mapping of the EcoRI Fragments of the Giant Linear Plasmid SCP1

A cosmid library was constructed for the 350-kb giant linear plasmid SCP1 and aligned on a successive linear map. Only a 0.8-kb gap has remained uncloned in the terminal inverted repeats close to both ends. Partial digestion of the aligned cosmids with EcoRI and hybridization with the flanking fragm...

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Autors principals: Redenbach, Matthias, Ikeda, Kazuya, Yamasaki, Masayuki, Kinashi, Haruyasu
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1998
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC107240/
https://ncbi.nlm.nih.gov/pubmed/9573173
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