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Cloning and Physical Mapping of the EcoRI Fragments of the Giant Linear Plasmid SCP1

A cosmid library was constructed for the 350-kb giant linear plasmid SCP1 and aligned on a successive linear map. Only a 0.8-kb gap has remained uncloned in the terminal inverted repeats close to both ends. Partial digestion of the aligned cosmids with EcoRI and hybridization with the flanking fragm...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Redenbach, Matthias, Ikeda, Kazuya, Yamasaki, Masayuki, Kinashi, Haruyasu
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1998
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107240/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9573173/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.10.2796-2799.1998
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