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Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions
In a gene targeting experiment, the generation of a targeting construct often requires complex DNA manipulations. We developed a set of cassettes and plasmids useful for creating targeting vectors to modify the mammalian genome. A positive selection marker cassette (PGK/EM7p-npt), which included dua...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1069132/ https://ncbi.nlm.nih.gov/pubmed/15784610 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni055 |
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