Use of GC Clamps in DHPLC Mutation Scanning

OBJECTIVE: Development of a systematic mutation detection assay strategy for denaturing high performance liquid chromatography (DHPLC). DESIGN: Adaptation of Guanine and Cytosine (GC)-clamping from denaturing gradient gel electrophoresis (DGGE) to DHPLC. METHODS: Three target sequences harboring kno...

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Detalhes bibliográficos
Main Authors: Wurzburger, Robert J., Gupta, Rajarsi, Parnassa, Andrew P., Jain, Sargam, Wexler, Jason A., Chu, Jia Li, Elkon, Keith B., Blank, Robert D.
Formato: Artigo
Idioma:Inglês
Publicado em: Marshfield Clinic Research Foundation 2003
Assuntos:
Original Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1069033/
https://ncbi.nlm.nih.gov/pubmed/15931297
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