Conversion of NfsA, the Major Escherichia coli Nitroreductase, to a Flavin Reductase with an Activity Similar to That of Frp, a Flavin Reductase in Vibrio harveyi, by a Single Amino Acid Substitution

NfsA is the major oxygen-insensitive nitroreductase of Escherichia coli, similar in amino acid sequence to Frp, a flavin reductase of Vibrio harveyi. Here, we show that a single amino acid substitution at position 99, which may destroy three hydrogen bonds in the putative active center, transforms N...

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Manylion Llyfryddiaeth

Cyhoeddwyd yn:	J Bacteriol
Prif Awduron:	Zenno, Shuhei, Kobori, Toshiro, Tanokura, Masaru, Saigo, Kaoru
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	American Society for Microbiology (ASM) 1998
Pynciau:	Enzymes and Proteins
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106901/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9440535/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.2.422-425.1998
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