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Conversion of NfsA, the Major Escherichia coli Nitroreductase, to a Flavin Reductase with an Activity Similar to That of Frp, a Flavin Reductase in Vibrio harveyi, by a Single Amino Acid Substitution

NfsA is the major oxygen-insensitive nitroreductase of Escherichia coli, similar in amino acid sequence to Frp, a flavin reductase of Vibrio harveyi. Here, we show that a single amino acid substitution at position 99, which may destroy three hydrogen bonds in the putative active center, transforms N...

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Detaylı Bibliyografya
Yayımlandı:J Bacteriol
Asıl Yazarlar: Zenno, Shuhei, Kobori, Toshiro, Tanokura, Masaru, Saigo, Kaoru
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology (ASM) 1998
Konular:
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106901/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9440535/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.2.422-425.1998
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