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One Hundred Seventy-Fold Increase in Excretion of an FV Fragment-Tumor Necrosis Factor Alpha Fusion Protein (sFV/TNF-α) from Escherichia coli Caused by the Synergistic Effects of Glycine and Triton X-100

To target tumor necrosis factor alpha (TNF-α) to tumor cells, recombinant DNA techniques were used to construct and express the fused gene V(K)LV(H)–TNF-α, which encodes the secreted form of single-chain fusion protein sFV/TNF-α in Escherichia coli. sFV/TNF-α was secreted into the culture medium and...

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Publicado no:Appl Environ Microbiol
Principais autores: Yang, Junbao, Moyana, Terence, MacKenzie, Samuel, Xia, Qun, Xiang, Jim
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1998
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106785/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9687443/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.8.2869-2874.1998
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