One Hundred Seventy-Fold Increase in Excretion of an FV Fragment-Tumor Necrosis Factor Alpha Fusion Protein (sFV/TNF-α) from Escherichia coli Caused by the Synergistic Effects of Glycine and Triton X-100
To target tumor necrosis factor alpha (TNF-α) to tumor cells, recombinant DNA techniques were used to construct and express the fused gene V(K)LV(H)–TNF-α, which encodes the secreted form of single-chain fusion protein sFV/TNF-α in Escherichia coli. sFV/TNF-α was secreted into the culture medium and...
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| Publicat a: | Appl Environ Microbiol |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1998
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106785/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9687443/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.8.2869-2874.1998 |
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