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Nucleic Acid (DNA, RNA) Quantification and RNA/DNA Ratio Determination in Marine Sediments: Comparison of Spectrophotometric, Fluorometric, and HighPerformance Liquid Chromatography Methods and Estimation of Detrital DNA

In this study, we compared three methods for extraction and quantification of RNA and DNA from marine sediments: (i) a spectrophotometric method using the diphenylamine assay; (ii) a fluorometric method utilizing selective fluorochromes (thiazole orange for total nucleic acids and Hoechst 33258 for...

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Bibliographische Detailangaben
Hauptverfasser: Dell’Anno, A., Fabiano, M., Duineveld, G. C. A., Kok, A., Danovaro, R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1998
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC106716/
https://ncbi.nlm.nih.gov/pubmed/9726866
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