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Nucleic Acid (DNA, RNA) Quantification and RNA/DNA Ratio Determination in Marine Sediments: Comparison of Spectrophotometric, Fluorometric, and HighPerformance Liquid Chromatography Methods and Estimation of Detrital DNA

In this study, we compared three methods for extraction and quantification of RNA and DNA from marine sediments: (i) a spectrophotometric method using the diphenylamine assay; (ii) a fluorometric method utilizing selective fluorochromes (thiazole orange for total nucleic acids and Hoechst 33258 for...

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Autori principali: Dell’Anno, A., Fabiano, M., Duineveld, G. C. A., Kok, A., Danovaro, R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 1998
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC106716/
https://ncbi.nlm.nih.gov/pubmed/9726866
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