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Rapid Fractionation of Wheat Leaf Protoplasts Using Membrane Filtration : The Determination of Metabolite Levels in the Chloroplasts, Cytosol, and Mitochondria

A technique is presented for measuring the in vivo metabolite levels in the chloroplast stroma, the cytosol, and the mitochondrial matrix of wheat (Triticum aestivum, var `Timmo') leaf protoplasts, in which membrane filtration is used to prepare fractions enriched in the different subcellular f...

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Detalles Bibliográficos
Main Authors: Lilley, Ross McC., Stitt, Mark, Mader, Gerhard, Heldt, Hans W.
Formato: Artigo
Idioma:Inglês
Publicado: 1982
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065808/
https://ncbi.nlm.nih.gov/pubmed/16662652
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