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Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa
A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His(6)-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro producti...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology
2005
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1065158/ https://ncbi.nlm.nih.gov/pubmed/15746370 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1642-1647.2005 |
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