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Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa

A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His(6)-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro producti...

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Autori principali: Woodyer, Ryan, Simurdiak, Michael, van der Donk, Wilfred A., Zhao, Huimin
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065158/
https://ncbi.nlm.nih.gov/pubmed/15746370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1642-1647.2005
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