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A Simple Method for Estimating Intactness of Spinach Leaf Protoplasts by Glycolate Oxidase Assay

A method was developed for the quantitative analysis of intactness of spinach leaf protoplasts using glycolate oxidase activity as an index. Since glycolate does not penetrate into protoplasts at neutral pH, the increase of O(2) consumption by the addition of glycolate to protoplast suspension was d...

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Main Authors: Nishimura, Mikio, Douce, Roland, Akazawa, Takashi
格式: Artigo
語言:Inglês
出版: 1985
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1064732/
https://ncbi.nlm.nih.gov/pubmed/16664242
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