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Translocation of Photosynthates into Vacuoles in Spinach Leaf Protoplasts

A method was developed for the isolation of vacuoles from the mesophyll protoplasts of spinach leaf, employing the discontinuous Ficoll density gradient centrifugation technique. Isolated vacuole preparations were judged to be free from other organellar fractions based on the assays of marker enzyme...

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Hlavní autoři: Asami, Sumio, Hara-Nishimura, Ikuko, Nishimura, Mikio, Akazawa, Takashi
Médium: Artigo
Jazyk:Inglês
Vydáno: 1985
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1064641/
https://ncbi.nlm.nih.gov/pubmed/16664172
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