A Fluorescent Gram Stain for Flow Cytometry and Epifluorescence Microscopy

The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organ...

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Main Authors: Mason, David J., Shanmuganathan, Subo, Mortimer, Fiona C., Gant, Vanya A.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1998
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Methods
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC106444/
https://ncbi.nlm.nih.gov/pubmed/9647848
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