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A Fluorescent Gram Stain for Flow Cytometry and Epifluorescence Microscopy

The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organ...

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Udgivet i:Appl Environ Microbiol
Principais autores: Mason, David J., Shanmuganathan, Subo, Mortimer, Fiona C., Gant, Vanya A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1998
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Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106444/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9647848/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.7.2681-2685.1998
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