Method Detection Limits of PCR and Immunofluorescence Assay for Cryptosporidium parvum in Soil

We determined and compared the method detection limits (MDL(α)) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDL(α) and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soil stud...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Walker, Mark J., Montemagno, Carlo, Bryant, Juliet C., Ghiorse, William C.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1998
Schlagworte:	Methods
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106315/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9603851/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.6.2281-2283.1998
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!