Method Detection Limits of PCR and Immunofluorescence Assay for Cryptosporidium parvum in Soil

We determined and compared the method detection limits (MDL(α)) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDL(α) and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soil stud...

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izdano v:	Appl Environ Microbiol
Principais autores:	Walker, Mark J., Montemagno, Carlo, Bryant, Juliet C., Ghiorse, William C.
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1998
Teme:	Methods
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106315/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9603851/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.6.2281-2283.1998
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