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Isolation of Guard Cell Protoplasts from Mechanically Prepared Epidermis of Vicia faba Leaves
A method for isolating guard cell protoplasts (GCP) from mechanically prepared epidermis of Vicia faba is described. Epidermis was prepared by homogenizing leaves in a Waring blender in a solution of 10% Ficoll, 5 millimolar CaCl(2), and 0.1% polyvinylpyrrolidone 40 (PVP). Attached mesophyll and epi...
Tallennettuna:
| Päätekijät: | , , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
1989
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1061900/ https://ncbi.nlm.nih.gov/pubmed/16666940 |
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