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Isolation of Guard Cell Protoplasts from Mechanically Prepared Epidermis of Vicia faba Leaves

A method for isolating guard cell protoplasts (GCP) from mechanically prepared epidermis of Vicia faba is described. Epidermis was prepared by homogenizing leaves in a Waring blender in a solution of 10% Ficoll, 5 millimolar CaCl(2), and 0.1% polyvinylpyrrolidone 40 (PVP). Attached mesophyll and epi...

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Bibliografske podrobnosti
Main Authors: Kruse, Tamara, Tallman, Gary, Zeiger, Eduardo
Format: Artigo
Jezik:Inglês
Izdano: 1989
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1061900/
https://ncbi.nlm.nih.gov/pubmed/16666940
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