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Isolation of Fixed and Viable Eggs, Central Cells, and Embryo Sacs from Ovules of Plumbago zeylanica
Three alternative protocols for light microscopy, electron microscopy, and biochemical characterization of isolated megagametophytic tissues are described employing enzymic maceration and microdissection of living and fixed ovular tissue of Plumbago zeylanica. Morphologically well preserved megagame...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1989
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1061665/ https://ncbi.nlm.nih.gov/pubmed/16666774 |
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